Difference between revisions of "Part:BBa K3376011"
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<partinfo>BBa_K3376011 short</partinfo> | <partinfo>BBa_K3376011 short</partinfo> | ||
| − | Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response with the control. | + | Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli Nissle strain (EcN) was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control. |
[[File:T--Mingdao--ww5.png|600px|center]] | [[File:T--Mingdao--ww5.png|600px|center]] | ||
Latest revision as of 18:35, 6 October 2020
tpxp-KatG-Tr/pSB1C3
Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli Nissle strain (EcN) was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 424
Illegal BglII site found at 1359
Illegal BglII site found at 1486
Illegal BamHI site found at 1276 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]