Difference between revisions of "Part:BBa K3570021"
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<h2>Usage</h2>
 
<h2>Usage</h2>
 
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The development of biotechnology for the industry pushed it to increase the productivity and safety of the biotechnological processes. One of those breakthroughs were the inducible promoters. The principle is based on the recruitment of transcriptional regulators using small-molecule responsive DNA-binding proteins (Janicki et al., 2004).</p>  
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The development of biotechnology for the industry pushed it to increase the productivity and safety of the biotechnological processes. One of those breakthroughs were the inducible promoters. The principle is based on the recruitment of transcriptional regulators using small-molecule responsive DNA-binding proteins (Janicki et al., 2004). However, chemically-induced systems present many drawbacks such as an uncontrolled spatiotemporal resolution and the speed of the cellular uptake and release of the inducer.</p>
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Revision as of 18:13, 6 October 2020


NLS-VP16-EL222 photosensitive transcription factor

Usage

The development of biotechnology for the industry pushed it to increase the productivity and safety of the biotechnological processes. One of those breakthroughs were the inducible promoters. The principle is based on the recruitment of transcriptional regulators using small-molecule responsive DNA-binding proteins (Janicki et al., 2004). However, chemically-induced systems present many drawbacks such as an uncontrolled spatiotemporal resolution and the speed of the cellular uptake and release of the inducer.

References

  • Janicki, S. M., Tsukamoto, T., Salghetti, S. E., Tansey, W. P., Sachidanandam, R., Prasanth, K. V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R. H., & Spector, D. L. (2004). From Silencing to Gene Expression. Cell, 116(5), 683–698. https://doi.org/10.1016/s0092-8674(04)00171-0

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]