Difference between revisions of "Part:BBa S04059"

Revision as of 17:42, 6 October 2020

katG (LAA).B0015

Construction intermediate

Characterization by team Mingdao 2020

ldhp-AQP-SpxB-Tr-tpxp-KatG-Tr/pSB1C3 (BBa_K3376012)

Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 228
Illegal BglII site found at 1163
Illegal BglII site found at 1290
Illegal BamHI site found at 1080
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 ==== Characterization by team Mingdao 2020 ====
-;tpxp-KatG-Tr/pSB1C3
-Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.
+;ldhp-AQP-SpxB-Tr-tpxp-KatG-Tr/pSB1C3 (BBa_K3376012)
+:Catalase converts H2O2 to O2 and H2O preventing cells from oxidative stress. When E. coli was expressing aquaporin (AQP) and pyruvate oxidase (SpxB) to produce H2O2, the cell growth was inhibited remarkably compared to a vector-only control. By adding of catalase (KatG), the growth rate became normal and have similar response to glucose concentrations with the control.
 [[File:T--Mingdao--ww5.png|600px|center]]