Difference between revisions of "Part:BBa K3408002"
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We would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could suicide.  
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We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could suicide.  
  
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
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We modifed our Trigger RNA with some bps in the 5’ terminal to increase its stability.
  
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>

Revision as of 15:45, 6 October 2020

We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could suicide.

We modifed our Trigger RNA with some bps in the 5’ terminal to increase its stability.

T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)