Difference between revisions of "Part:BBa K3408002"
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We would use the trigger NRA to release mazF when switch-mazF is expressed in earthworm’s faeces. So our engineeringengineered bacterium could suicidesuicide. We modifed our trigger RNA with some bps in the 5’ terminal the same as the yellow part to increase its stability.
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We would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could suicide.  
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We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.
  
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>

Revision as of 07:46, 6 October 2020

We would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could suicide.

We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.

T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)