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Revision as of 05:44, 6 October 2020


Switch RNA(of toehold)

It is the part of toehold switch which functions as the controller of suicide switch. It is activated by Pci when oxygen exists in wormcast.

We hope our engineering bacteria not express of Dpnc accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the PCI downstream

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]