Difference between revisions of "Part:BBa K3570018"

 
Line 1: Line 1:
  
 +
__NOTOC__
 +
<partinfo>BBa_K3570018 short</partinfo>
 +
 +
The MET17 gene, found in the <i>Saccharomyces cerevisiae</i> yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism; catalyses the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acids; localized to the cytoplasm and plasma membrane.
 +
 +
When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the wanted plasmid.
 +
 +
References:
 +
1- SGD:S000004294
 +
 +
2- Masselot, M., & de Robichon-Szulmajster, H. (1975). Methionine biosynthesis in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 139(2), 121–132. https://doi.org/10.1007/bf00264692
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K3570018 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K3570018 parameters</partinfo>
 +
<!-- -->

Revision as of 05:28, 6 October 2020


MET17 selection marker

The MET17 gene, found in the Saccharomyces cerevisiae yeast, encodes a bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism; catalyses the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acids; localized to the cytoplasm and plasma membrane.

When inserted into an integrative or replicative plasmid, MET17 allows to counter-select the cells that acquired the wanted plasmid.

References: 1- SGD:S000004294

2- Masselot, M., & de Robichon-Szulmajster, H. (1975). Methionine biosynthesis in Saccharomyces cerevisiae. Molecular and General Genetics MGG, 139(2), 121–132. https://doi.org/10.1007/bf00264692

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 378
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]