Difference between revisions of "Part:BBa K3376001"

Revision as of 05:05, 6 October 2020

GFP-Tr/pSB1C3

The basic part of GFPmut1 was modified from GFPmut1 (Part:BBa_K1159311) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression. The GFPmut1 was assembled with a double terminator.

Expression

GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.

Transformation

The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3376001 short</partinfo>
+The basic part of GFPmut1 was modified from GFPmut1 ([https://parts.igem.org/Part:BBa_K1159311 Part:BBa_K1159311]) designed by TU-Munich in iGEM 2013 by adding ATG and a stop codon for protein expression. The GFPmut1 was assembled with a double terminator.
+=== Expression ===
+GFP was detected at Ex/Em = 483/513. The fluorescence expression levels were measured for lactate dehydrogenase promoter (ldhp) [ldhp-GFP-Tr/pSB1C3 (BBa_K3376002)] and thiol peroxidase promoter (tpxp) [tpxp-GFP-Tr/pSB1C3 (BBa_K3376004)] activity of S. mutans.
+[[File:T--Mingdao--ww1.png|450px|center]]
+=== Transformation ===
+The ldhp-GFP-Tr/pSB1C3 [BBa_K3376002] was transformed into E. coli Nissle strain. The high expression of green fluorescent protein was observed under a blue led light.
+[[File:T--Mingdao--ww2.png|600px|center]]
 <!-- Add more about the biology of this part here