Difference between revisions of "Part:BBa K3453132"
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<partinfo>BBa_K3453132 short</partinfo> | <partinfo>BBa_K3453132 short</partinfo> | ||
| − | This part is an sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) expression cassette under the control of the DmRbcL Toehold Switch 1.2 ([[Part:BBa K3453032|BBa K3453032]]). | + | This part is an sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) expression cassette under the control of the DmRbcL Toehold Switch 1.2 ([[Part:BBa K3453032|BBa K3453032]]) for sequence-based detection of the rosewood species ''Dalbergia maritima'' var. ''pubescens''. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:24, 4 October 2020
DmRbcL Toehold Switch 1.2 with sfGFP-LVAtag
This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the DmRbcL Toehold Switch 1.2 (BBa K3453032) for sequence-based detection of the rosewood species Dalbergia maritima var. pubescens.
Usage and Biology
In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by the synthetic DmRbcL Toehold Switch 1.2 (BBa K3453032) and placed under the control of the T7 promoter (BBa_K2150031) and of the strong SBa_000587 synthetic terminator (BBa_K3453000).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]