Difference between revisions of "Part:BBa K3453153"

Revision as of 10:14, 4 October 2020

DmTrnL-UAA Toehold Switch 1.3 with sfGFP-LVAtag

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the DmTrnL-UAA Toehold Switch 1.3 (BBa K3453053).

Usage and Biology

In this composite part sfGFP-LVAtag (BBa_K2675006) was equipped by the synthetic DmTrnL-UAA Toehold Switch 1.3 (BBa K3453053) and placed under the control of the T7 promoter (BBa_K2150031) and of the strong SBa_000587 synthetic terminator (BBa_K3453000).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3453153 short</partinfo>
-to do
+This part is an sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) expression cassette under the control of the DmTrnL-UAA Toehold Switch 1.3 ([[Part:BBa K3453053|BBa K3453053]]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In this composite part sfGFP-LVAtag ([[Part:BBa_K2675006|BBa_K2675006]]) was equipped by the synthetic DmTrnL-UAA Toehold Switch 1.3 ([[Part:BBa K3453053|BBa K3453053]]) and placed under the control of the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and of the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
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