Difference between revisions of "Part:BBa K3408003"

Line 6: Line 6:
  
 
We hope our engineering bacteria not express of Dpnc accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the PCI downstream
 
We hope our engineering bacteria not express of Dpnc accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the PCI downstream
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:31, 4 October 2020


Switch RNA(of toehold)

It is the part of toehold switch which functions as the controller of suicide switch. It is activated by Pci when oxygen exists in wormcast.

We hope our engineering bacteria not express of Dpnc accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the Switch RNA to mazF, following the PCI downstream Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]