Difference between revisions of "Part:BBa K103001"

Latest revision as of 01:01, 28 October 2008

AID protein

AID (Activation Induced Deaminase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.

We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 78
Illegal SapI site found at 179

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-*AID (Activation Induced Deamidase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
+*AID (Activation Induced Deaminase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
 *We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.
 |[[Image:Aid.jpg|250px]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
 |}
 <span class='h3bb'>Sequence and Features</span>