Difference between revisions of "Part:BBa K103001"
(→‎Usage and Biology)
 
(3 intermediate revisions by one other user not shown)
Line 6: Line 6:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
*AID (Activation Induced Deamidase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
+
*AID (Activation Induced Deaminase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
  
 
*We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.
 
*We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.
  
−
|[[Image:Aid.jpg]]
+
|[[Image:Aid.jpg|250px]]
 +
This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
 
|}
 
|}
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 01:01, 28 October 2008

AID protein

Usage and Biology

  • AID (Activation Induced Deaminase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
  • We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.
 Aid.jpg

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 78
    Illegal SapI site found at 179