Difference between revisions of "Part:BBa K103014"

Revision as of 00:42, 28 October 2008

OmpA_linker_alpha_linker

It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit alpha ( BBa_K103008)
Fusion partners are connected via Gly-Ser-Gly linker
It's base for creation of hunter fusions in 'hunter and prey' selection system
Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
Interaction between hunter and prey (fused with BBa_K103020) makes cells ampicillin resistant
This part uses our NdeI/SacI/BamHI fusion cloning substandard

This protein structure was predicted using threading and protein fold prediction and may differ from actual one.

Sequence and Features

Assembly Compatibility:

@@ Line 16: / Line 16: @@
 *This part uses our NdeI/SacI/BamHI fusion cloning substandard
 |[[Image:Ompalinkalfa.jpg|250px]]
+This protein structure was predicted using threading and protein fold prediction and may differ from actual one.
 |}