Difference between revisions of "Part:BBa K3651034"
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Latest revision as of 20:19, 3 October 2020
Constitutive expressor of Ferric Uptake Regulator Protein
J23100-FUR. This is a part that constitutively expresses Ferric Uptake Regulator Protein (FUR). In the presence of iron, iron-bound FUR can bind to a FUR box and block transcription. These FUR boxes are typically within promoters. We could use this part in conjunction with pfur1-mcherry (BBa_K3651026) to confer iron-sensitive expression of mCherry.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 183
Illegal XhoI site found at 368 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 323
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 104
Illegal BsaI.rc site found at 320