Difference between revisions of "Part:BBa K3570008"

Revision as of 18:03, 3 October 2020

HIS3 selection marker

The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.

When inserted into an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.

Sources 1- RS313 plasmid https://www.addgene.org/vector-database/3972/

2- https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3

Sequence and Features