Difference between revisions of "Part:BBa K3588003"
| Line 18: | Line 18: | ||
K<sub>cat</sub> = 2600 min<sup>-1</sup> | K<sub>cat</sub> = 2600 min<sup>-1</sup> | ||
K<sub>M</sub> = 2.9 μM | K<sub>M</sub> = 2.9 μM | ||
| + | |||
| + | <h5>other details</h5> | ||
| + | half life (at 37°C) = ~ 6hrs | ||
===usage=== | ===usage=== | ||
Revision as of 17:05, 3 October 2020
Cocaine Esterase mutant enzyme (E172-173)
The mutant enzyme of part (part:BBa_K3588001). It is able to breakdown cocaine, as well as benzoylecogonine, through hydrolysis into benzoate and ecgonine methyl ester. With a dramatically increased in vitro half life from the wildtype enzyme it is currently in phase II trials as a cocaine overdose treatment,[1] though in our project we were intending to use it to breakdown cocaine in the sewers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1179
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]