Difference between revisions of "Part:BBa K3588003"
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<partinfo>BBa_K3588003 short</partinfo> | <partinfo>BBa_K3588003 short</partinfo> | ||
| − | The mutant enzyme of part ([[part:BBa_K3588001]]). It is able to breakdown cocaine, as well as benzoylecogonine, through hydrolysis into benzoate and ecgonine methyl ester. With a dramatically increased <i>in vitro<i> half life from the wildtype enzyme it is currently in phase II trials as a cocaine overdose treatment,<ref>https://pubs.acs.org/doi/abs/10.1021/cb500257s</ref> though in our project we were intending to use it to breakdown cocaine in the sewers. | + | The mutant enzyme of part ([[part:BBa_K3588001]]). It is able to breakdown cocaine, as well as benzoylecogonine, through hydrolysis into benzoate and ecgonine methyl ester. With a dramatically increased <i>in vitro</i> half life from the wildtype enzyme it is currently in phase II trials as a cocaine overdose treatment,<ref>https://pubs.acs.org/doi/abs/10.1021/cb500257s</ref> though in our project we were intending to use it to breakdown cocaine in the sewers. |
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Revision as of 16:31, 3 October 2020
Cocaine Esterase mutant enzyme (E172-173)
The mutant enzyme of part (part:BBa_K3588001). It is able to breakdown cocaine, as well as benzoylecogonine, through hydrolysis into benzoate and ecgonine methyl ester. With a dramatically increased in vitro half life from the wildtype enzyme it is currently in phase II trials as a cocaine overdose treatment,[1] though in our project we were intending to use it to breakdown cocaine in the sewers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1179
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]