Difference between revisions of "Part:BBa K2290003"

 
 
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<partinfo>BBa_K2290003 short</partinfo>
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<partinfo>BBa K2290003 short</partinfo>
  
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T7 promoter for Gibson overhang and IPTG induced expression
 
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===Usage and Biology===
 
===Usage and Biology===
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===Contribution: New documentation from Evry_Paris-Saclay 2020===
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This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG at 3' end.
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The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].
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==References==
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[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2290003 SequenceAndFeatures</partinfo>
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<partinfo>BBa K2290003 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2290003 parameters</partinfo>
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<partinfo>BBa K2290003 parameters</partinfo>
 
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Latest revision as of 14:20, 3 October 2020


No part name specified with partinfo tag.

Usage and Biology

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG at 3' end.

The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].

References

[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.


Sequence and Features No part name specified with partinfo tag.