Difference between revisions of "Part:BBa K3408000:Design"

Revision as of 13:34, 3 October 2020

promoter nar(fnr regulated)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The spacer sequence of 15 bp between the - 35 and - 10 elements of the upstream region of the wild-type nar promoter was randomized.

Source

The sequence is found through the paper <Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli>.

References

Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299.

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	===References===		===References===
		+	Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299.