Difference between revisions of "Part:BBa K3610004:Design"

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===Design Notes===
 
===Design Notes===
−
Mustn't contain BsaI restriction sites.
+
Codon optimized for C. reinhardtii. This sequence doesn't contain codons with a occurrence probability lower than 10%.
−
 
+
  
 +
The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.
  
 
===Source===
 
===Source===
  
−
Ordered from IDT
+
Cyril Zipfel Lab at UZH, Switzerland
 +
Codon optimized with SnapGene
  
 
===References===
 
===References===
 +
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
 +
 +
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
 +
 +
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
 +
 +
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.
 +
 +
Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.
 +
 +
Naya, Hugo; Romero, Héctor; Carels, Nicola; Zavala, Alejandro; Musto, Héctor (2001): Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii. In: FEBS Letters 501 (2), S. 127–130. DOI: 10.1016/S0014-5793(01)02644-8.

Latest revision as of 13:03, 3 October 2020


BAK1 ectodomain from Arabidopsis thaliana - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 778
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 708
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for C. reinhardtii. This sequence doesn't contain codons with a occurrence probability lower than 10%.

The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.

Source

Cyril Zipfel Lab at UZH, Switzerland Codon optimized with SnapGene

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

Zhou, Qi; Liu, Jun; Wang, Jingyi; Chen, Sufen; Chen, Lijuan; Wang, Jinfa et al. (2020): The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling. In: Journal of integrative plant biology 62 (5), S. 556–562. DOI: 10.1111/jipb.12847.

Naya, Hugo; Romero, Héctor; Carels, Nicola; Zavala, Alejandro; Musto, Héctor (2001): Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii. In: FEBS Letters 501 (2), S. 127–130. DOI: 10.1016/S0014-5793(01)02644-8.