Difference between revisions of "Part:BBa K3610002:Design"

 
(→‎Source)
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===Source===
 
===Source===
  
−
PCR amplified from the BAK1 sequence.
+
Cyril Zipfel Lab at UZH, Switzerland
  
 
===References===
 
===References===

Revision as of 18:18, 30 September 2020


BAK1 from Arabidopsis thaliana without signal sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal sequence was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.


Source

Cyril Zipfel Lab at UZH, Switzerland

References