Difference between revisions of "Part:BBa K3349001:Design"

Revision as of 17:24, 29 September 2020

Pectin Lyase PelB

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 261
Illegal BamHI site found at 1146
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus based on previous pectin lyase studies [4].

Source

The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2.Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.

3. Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R. and Schwede, T.(2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296-W303.

4. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81

@@ Line 7: / Line 7: @@
 ===Design Notes===
-The part was codon optimized for use in <i> E. coli </i>. The his tag was placed on the C-terminus based on previous pectin lyase studies [1]
+The part was codon optimized for use in <i> E. coli </i>. The his tag was placed on the C-terminus based on previous pectin lyase studies [4].
 ===Source===