Difference between revisions of "Part:BBa K3349001"
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<partinfo>BBa_K3349001 short</partinfo> | <partinfo>BBa_K3349001 short</partinfo> | ||
| − | This Pectin Lyase is a hypothetical protein from <i> Paenibaciullus amylolyticus </i>. | + | This Pectin Lyase is a hypothetical protein from <i> Paenibaciullus amylolyticus </i>. With Pnl and PelC, PelB is able to cleave and therefore breakdown pectin or homogalacturonan substrates. This protein has been characterized to cleave methylated pectin (at unmethylated sites) and polygalacturonic acid [2]. A hexahistidine tag was added onto the C-terminus for nickel affinity purification. |
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Revision as of 17:19, 29 September 2020
Pectin Lyase PelB
This Pectin Lyase is a hypothetical protein from Paenibaciullus amylolyticus . With Pnl and PelC, PelB is able to cleave and therefore breakdown pectin or homogalacturonan substrates. This protein has been characterized to cleave methylated pectin (at unmethylated sites) and polygalacturonic acid [2]. A hexahistidine tag was added onto the C-terminus for nickel affinity purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 261
Illegal BamHI site found at 1146 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]