Difference between revisions of "Part:BBa K3349000"
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For building thermostable variants of this Pnl enzyme we first did some homology modelling as the crystal structure has not been determined. | For building thermostable variants of this Pnl enzyme we first did some homology modelling as the crystal structure has not been determined. | ||
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Revision as of 16:49, 29 September 2020
Pectin Lyase Pnl
This Pectin Lyase is from the organism Paenibacillus amylolyticus . Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. A hexa-histidine tag was added to the C-terminus of the coding sequence for nickel affinity purification.
For building thermostable variants of this Pnl enzyme we first did some homology modelling as the crystal structure has not been determined.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]