Difference between revisions of "Part:BBa K3349000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence was codon optimized for <i>E. coli </i>. The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [ | + | The sequence was codon optimized for <i>E. coli </i>. The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [2]. |
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===Source=== | ===Source=== | ||
Revision as of 19:12, 28 September 2020
Pectin Lyase Pnl
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [2].
Source
Sequence was taken from the Uniprot database for synthesis. The sequence ID from NCBI is KAA8782864.1.
References
1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81