Difference between revisions of "Part:BBa K3349001:Design"

 
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===References===
 
===References===
 +
1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from <i> Bacillus haldurans </i> M29.

Revision as of 14:57, 23 September 2020


Pectin Lyase PelB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 261
    Illegal BamHI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus based on previous pectin lyase studies [1]


Source

The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.

References

1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from Bacillus haldurans M29.