Difference between revisions of "Part:BBa C0053"

Revision as of 01:49, 30 April 2006

c2 repressor from Salmonella phage P22 (+LVA)

The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.

Usage and Biology

P22 c2 is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type c2 to contain an LVA tag,

and two TAA stop codons.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 166

Functional Parameters

direction	Forward
function	Rep
ligands	?
protein	C2 P22
swisspro	P69202
tag	LVA

@@ Line 3: / Line 3: @@
 <partinfo>BBa_C0053 short</partinfo>
-The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053.      The sequence contains a LVA tag for faster degredation.</p>
+The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053.      The sequence contains a LVA tag for faster degredation.
 ===Usage and Biology===
-P22 c2 is a member of the lamboid cI protein family.<br>     The coding sequence has been modified from wild type c2 to contain an LVA      tag,<br>     and two TAA stop codons.
+P22 c2 is a member of the lamboid cI protein family.
+The coding sequence has been modified from wild type c2 to contain an LVA      tag,
+and two TAA stop codons.