Difference between revisions of "Part:BBa K3349000:Design"

 
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===References===
 
===References===
 +
1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from <i> Bacillus haldurans </i> M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81

Revision as of 14:50, 23 September 2020


Pectin Lyase Pnl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [1].


Source

Sequence was taken from the Uniprot database for synthesis. The sequence ID from NCBI is KAA8782864.1.

References

1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81