Difference between revisions of "Part:BBa K3498004"

(AbrB protein inhibits B. subtilis sporulation)
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AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of
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numerous that is present in vegetatively growing cells and is inactivated or eliminated by
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__NOTOC__
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<partinfo>BBa_K3317007 short</partinfo>
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Sensitive promoter against protocatechuic acid and presents its maximum induction in the presence of 1000mM RPU 1.6 of vanillinic acid with a leak of 4.5x10-3  RPU in absence of induction(Meyer, A. J <i> et. al</i> 2019) this part needs its regulatory protein (<partinfo>K3317005 </partinfo>)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3317007 SequenceAndFeatures</partinfo>
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AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous that is present in vegetatively growing cells and is inactivated or eliminated by
 
the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding
 
the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding
 
protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm
 
protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm
 
and sporulation formation in B. subtilis, it affects the production by binding into DNA and
 
and sporulation formation in B. subtilis, it affects the production by binding into DNA and
 
blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017).
 
blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017).

Revision as of 05:40, 22 September 2020


Inducible p3B5C promoter

Sensitive promoter against protocatechuic acid and presents its maximum induction in the presence of 1000mM RPU 1.6 of vanillinic acid with a leak of 4.5x10-3 RPU in absence of induction(Meyer, A. J et. al 2019) this part needs its regulatory protein (BBa_K3317005)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


AbrB protein inhibits B. subtilis sporulation. The AbrB protein of the spore-forming bacterium Bacillus subtilis is a repressor of numerous that is present in vegetatively growing cells and is inactivated or eliminated by the action of Spo0A at the end of the exponential phase of growth; AbrB is a DNA-binding protein that acts by repressing target genes (Banse et al., 2008) . This part can be used for inhibiting biofilm and sporulation formation in B. subtilis, it affects the production by binding into DNA and blocking transcription. It affects more than +100 genes as it is a major regulator (Zhu & Stülke, 2017).