Difference between revisions of "Part:BBa C0052"

Revision as of 01:49, 30 April 2006

cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.

Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag,

two TAA stop codons, and its EcoRI recognition site has been mutated out

by changing base 36 from T to C to be BioBrick-compatible.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 688
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

direction	Forward
function	Rep
ligands	RecA?
protein	CI 434-LVA
swisspro	P16117
tag	LVA

@@ Line 3: / Line 3: @@
 <partinfo>BBa_C0052 short</partinfo>
-The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052.      The sequence contains a LVA tag for faster degredation and has no RBS.</p>
+The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052.      The sequence contains a LVA tag for faster degredation and has no RBS.
 ===Usage and Biology===
-cI is a member of the lamboid cI protein family.<br>     The coding sequence has been modified from wild type cI to contain an LVA      tag,<br>  two TAA stop codons, and its EcoRI recognition site has been mutated out<br>     by changing base 36 from T to C to be BioBrick-compatible.
+cI is a member of the lamboid cI protein family.
+The coding sequence has been modified from wild type cI to contain an LVA      tag,
+two TAA stop codons, and its EcoRI recognition site has been mutated out
+by changing base 36 from T to C to be BioBrick-compatible.