Difference between revisions of "Part:BBa K3610015:Design"
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Revision as of 16:15, 13 September 2020
LargeBit NanoLuc - codon optimized for C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.
Source
The sequence was taken of Part:BBa_K3168002 from the iGEM registry and then codon optimized with SnapGene.