Difference between revisions of "Part:BBa K3610008:Design"
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Revision as of 14:44, 8 September 2020


EFR ectodomain - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons that showed a codon frequency of less than 5% were changed into codons preferred by C. reinhardtii. If the preferred codon was interfering with the RFC standards, a codon with a lower codon frequency was chosen.


Source

Ordered from IDT

References