Difference between revisions of "Part:BBa K3447002"

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<partinfo>BBa_K3447002 short</partinfo>
 
<partinfo>BBa_K3447002 short</partinfo>
  
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the <i>Enterobacteriaceae</i> family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
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The <i>cvaC</i> gene produces colicin V, which is also called ColV, is a type of bacteriocin secreted by <i>E. coli</i>. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein, which is synthesized by <i>cvi</i> gene.
 
The <i>cvaC</i> gene produces colicin V, which is also called ColV, is a type of bacteriocin secreted by <i>E. coli</i>. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein, which is synthesized by <i>cvi</i> gene.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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Colicin V is a non-ribosome-encoded antibacterial peptide produced by many <i>E. coli</i> strains and other members of the <i>Enterobacteriaceae</i> family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of <i>E. coli</i>.<br><br>
 
We will use cvaC-cvi to express ColV and immunity protein, thereby killing "enemy" bacteria.<br>
 
We will use cvaC-cvi to express ColV and immunity protein, thereby killing "enemy" bacteria.<br>
  

Revision as of 04:00, 5 September 2020


xynD, which produces xylanase


The cvaC gene produces colicin V, which is also called ColV, is a type of bacteriocin secreted by E. coli. Besides, it has a killing effect on other closely related bacteria that cannot secrete specific colicin immunity protein, which is synthesized by cvi gene.


Usage and Biology

Colicin V is a non-ribosome-encoded antibacterial peptide produced by many E. coli strains and other members of the Enterobacteriaceae family, which kills bacteria by destroying the membrane potential of sensitive target cells. Due to the existence and composition of the outer membrane of Gram-negative bacteria, natural colicin V is usually inactive to it, but its expression in lactic acid bacteria has also been proposed in the literature: naturally generated colicin V can destroy sensitive targets cell membrane potential to kill Gram-positive bacteria. The gene encoding colicin V is derived from the pAPEC-O2-ColV plasmid of E. coli.

We will use cvaC-cvi to express ColV and immunity protein, thereby killing "enemy" bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712