Difference between revisions of "Part:BBa K3447000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | There is a repetitive sequence between <i> | + | There is a repetitive sequence between <i>cvaA</i> and <i>cvaB</i> gene, and other than that there is no need to pay attention. |
===Source=== | ===Source=== |
Revision as of 14:32, 4 September 2020
luxI, an autoinducer synthetase for AHL
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a repetitive sequence between cvaA and cvaB gene, and other than that there is no need to pay attention.
Source
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.
References
1. Coproduction of colicin V and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest., Journal of Applied Microbiology, 122, 1159-1167.
2. Colicin V can be produced by lactic acid bacteria.(1999), Letters in Applied Microbiology, 29, 37-41.
3. Mutational Analysis of Cvab, an ABC Transporter Involved in the Secretion of Active Colicin V., Kai-Hui Wu., Ying-Hsin Hsieh., Phang C. Tai, Plos One, 7(4), e35382.
4. Purification and Characterization of Colicin V from Escherichia coli Culture Supernatants.(1994), Biochemistry, 33, 6911-6917.