Difference between revisions of "Part:BBa K118019"

 
 
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<partinfo>BBa_K118019 short</partinfo>
 
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This is the coding sequence of glgC from ''Escherichia coli'' JM109 (see [https://parts.igem.org/wiki/index.php?title=Part:BBa_K118015 BBa_K118015]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.  
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This is the coding sequence of glgC from ''Escherichia coli'' JM109 (see [https://parts.igem.org/wiki/index.php?title=Part:BBa_K118015 BBa_K118015]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' for blue-white selection.  
  
 
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_K118019 parameters</partinfo>
 
<partinfo>BBa_K118019 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: 8.0 ± 8.3% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 22:46, 3 September 2020

Plac+rbs+glgC (ADP-glucose pyrophosphorylase)

This is the coding sequence of glgC from Escherichia coli JM109 (see BBa_K118015) with a ribosome binding site coupled to an upstream Lac promoter and lacZ' for blue-white selection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_K118019 parameters

Burden Imposed by this Part:

Burden Value: 8.0 ± 8.3%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.