Difference between revisions of "Part:BBa K143075"
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<partinfo>BBa_K143075 short</partinfo> | <partinfo>BBa_K143075 short</partinfo> | ||
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AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP proein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>. | AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP proein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>. | ||
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The amyE 3' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 5' amyE integration sequence(<bbpart>BBa_K143001</bbpart>) is cloned onto the 5' end of the construct. | The amyE 3' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 5' amyE integration sequence(<bbpart>BBa_K143001</bbpart>) is cloned onto the 5' end of the construct. | ||
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The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough. | The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
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<partinfo>BBa_K143075 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143075 SequenceAndFeatures</partinfo> | ||
Revision as of 18:15, 27 October 2008
B. subtilis AmyE locus GFP output promoter and RBS tester
AmyE 3' Integration sequence(BBa_K143002) coupled to GFP proein coding region and the registry double terminator (part BBa_I13401.
The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.
The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644