Difference between revisions of "Part:BBa K103014"

Revision as of 17:49, 27 October 2008

OmpA_linker_alpha_linker

It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit alpha ( BBa_K103008)
Fusion partners are connected via Gly-Ser-Gly linker
It's base for creation of hunter fusions in 'hunter and prey' selection system
Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
Interaction between hunter and prey (fused with BBa_K103020) makes cells ampicillin resistant
This part uses our NdeI/SacI/BamHI fusion cloning substandard

Sequence and Features

Assembly Compatibility:

@@ Line 6: / Line 6: @@
 <!-- Add more about the biology of this part here -->
+{|
+|
 ===Usage and Biology===
 *It's fusion of E. coli outer membrane protein (OmpA) domain ([[Part:BBa_K103006 | BBa_K103006]]) with beta-lactamase subunit alpha ([[Part:BBa_K103008 | BBa_K103008]])
@@ Line 13: / Line 15: @@
 *Interaction between hunter and prey (fused with [[Part:BBa_K103020 | BBa_K103020]]) makes cells ampicillin resistant
 *This part uses our NdeI/SacI/BamHI fusion cloning substandard
+|[[Image:Ompalinkalfa.jpg|250px]]
+|}