Difference between revisions of "Part:BBa K3458003:Design"
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===Source=== | ===Source=== | ||
+ | *The HQT gene we used comes from the genome of Lonicera japonica ([https://www.ncbi.nlm.nih.gov/nuccore/JF261014.1 GeneBank: JF261014.1]). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company. | ||
− | + | *The 35s promoter ([https://parts.igem.org/Part:BBa_K414002 BBa_K414002]) has been registered by the iGEM10_Nevada team. | |
===References=== | ===References=== |
Revision as of 07:56, 17 August 2020
35S Promoter + HQT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191
Design Notes
- The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of Oryza sativa L.. The promoter GluD-1 (BBa_K3458002) that we plan to use with specific expression characteristics in Oryza sativa L. endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part (BBa_K3458004) .
Source
- The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.
- The 35s promoter (BBa_K414002) has been registered by the iGEM10_Nevada team.