Difference between revisions of "Part:BBa K3458003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
'''Because this is a composite of promoter and the coding region for the HQT sequence, it can be expressed by its two components--please see [https://parts.igem.org/Part:BBa_K3458001 BBa_K3458001] and [https://parts.igem.org/Part:BBa_K414002 BBa_K414002].''' | '''Because this is a composite of promoter and the coding region for the HQT sequence, it can be expressed by its two components--please see [https://parts.igem.org/Part:BBa_K3458001 BBa_K3458001] and [https://parts.igem.org/Part:BBa_K414002 BBa_K414002].''' | ||
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+ | The flower buds of ''Lonicera japonica'' are widely used in Chinese medicine for their anti-inflammatory properties. The reason why ''L. japonica'' has potent and significant effects is that it contains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from ''L. japonica.''. | ||
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+ | [File:HQT_Pathway.png|none|thumb|650px| | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 07:27, 17 August 2020
35S Promoter + HQT
This part is the L. japonica. hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) coding region, with a composite 35s promoter element.
Usage and Biology
Because this is a composite of promoter and the coding region for the HQT sequence, it can be expressed by its two components--please see BBa_K3458001 and BBa_K414002.
The flower buds of Lonicera japonica are widely used in Chinese medicine for their anti-inflammatory properties. The reason why L. japonica has potent and significant effects is that it contains various active components, especially chlorogenic acid (CGA). This is a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT) gene encoding a protein of 439 amino acids from L. japonica..
[File:HQT_Pathway.png|none|thumb|650px|
Characterization
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191