Difference between revisions of "Part:BBa K3458004:Design"
(→Design Notes) |
|||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of rice. The promoter GluD-1 cannot drive the HQT express in Oryza sativa L. protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT ([https://parts.igem.org/Part:BBa_K3458004 BBa_K3458004]) composite element for comparison with this part. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Revision as of 03:34, 17 August 2020
GluD-1 Promoter + HQT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 225
Design Notes
The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of rice. The promoter GluD-1 cannot drive the HQT express in Oryza sativa L. protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT (BBa_K3458004) composite element for comparison with this part.
Source
A