Difference between revisions of "Part:BBa K3458001:Design"
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===Source=== | ===Source=== | ||
− | The HQT gene we used comes from the genome of ''Lonicera japonica'' (GeneBank:[https://www.ncbi.nlm.nih.gov/nuccore/JF261014.1 | + | The HQT gene we used comes from the genome of ''Lonicera japonica'' (GeneBank:[https://www.ncbi.nlm.nih.gov/nuccore/JF261014.1 JF261014.1]). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of ''Oryza sativa L.''.The HQT was synthesized in TIANYI HUIYUAN Company. |
===References=== | ===References=== |
Revision as of 02:33, 17 August 2020
HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- We synthesized this DNA sample through TIANYI HUIYUAN Company.
In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..
- In order to meet the assembly requirements, we removed the illegal restriction site.
- In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003).
Source
The HQT gene we used comes from the genome of Lonicera japonica (GeneBank:JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.