Difference between revisions of "Part:BBa K157035"

 
 
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__NOTOC__
 
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<partinfo>BBa_K157035 short</partinfo>
 
<partinfo>BBa_K157035 short</partinfo>
  
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP,  GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP(intracellular part).
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A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin,  GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP.
 
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Part was derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008.<br>
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For further information see the description of the parts mentioned above.<br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 17:34, 27 October 2008

SP-Lipocalin-GGGSlinker-Tm-nYFP

A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP. Part was derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008.
For further information see the description of the parts mentioned above.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal BamHI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12