Difference between revisions of "Part:BBa K899008:Design"

 
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===Design Notes===
 
===Design Notes===
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none
 
−
 
  
 +
It is worth noting that similar ccdA parts exist on the registry however their sequence do not match that of the part described.
  
 
===Source===
 
===Source===
  
−
This antitoxin is found in E. coli and the sequence is derived from NCBI
+
The ccdA antitoxin is found natively in E.coli with sequence obtained from the pCoo plasmid (NCBI Accession Number: NC_007635.1). This was confirmed with sequence from NCBI Accession Number: NC_019000.
  
 
===References===
 
===References===
 +
 +
Bernard P., Couturier M. 1992. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J. Mol. Biol. 226: p735–745.
 +
 +
Vandervelde A., Drobnak I., Hadži S., Sterckx Y.GJ., Welte T., Greve. H.D., Charlier D., Efremov R., Loris R., Lah J. 2017. Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. NAR. 45(6): p2937-2950.

Latest revision as of 15:23, 8 August 2020


antitoxin ccdA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is worth noting that similar ccdA parts exist on the registry however their sequence do not match that of the part described.

Source

The ccdA antitoxin is found natively in E.coli with sequence obtained from the pCoo plasmid (NCBI Accession Number: NC_007635.1). This was confirmed with sequence from NCBI Accession Number: NC_019000.

References

Bernard P., Couturier M. 1992. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes. J. Mol. Biol. 226: p735–745.

Vandervelde A., Drobnak I., Hadži S., Sterckx Y.GJ., Welte T., Greve. H.D., Charlier D., Efremov R., Loris R., Lah J. 2017. Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. NAR. 45(6): p2937-2950.