Difference between revisions of "Part:BBa K3634017:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In the initial sequence obtained from Tabor's pJT122 plasmid, an illegal EcoR1 site is found. Uppsala-Sweden iGEM 2011 removed the site by carrying out point mutagenesis of A4C and A6C to obtain the part BBa_K592002. The part sequence was then subject to codon optimisation using the IDT codon optimisation tool to allow optimum expression of ccaR in the E.coli chassis. No new illegal restriction sites were introduced by this step. | + | In the initial sequence obtained from Tabor's pJT122 plasmid, an illegal EcoR1 site is found. Uppsala-Sweden iGEM 2011 removed the site by carrying out point mutagenesis of A4C and A6C to obtain the part BBa_K592002. The part sequence was then subject to codon optimisation using the IDT codon optimisation tool to allow optimum expression of ccaR in the E.coli chassis. No new illegal restriction sites were introduced by this step. |
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===Source=== | ===Source=== | ||
Revision as of 14:49, 8 August 2020
ccaR (Codon Optimised for E.coli)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the initial sequence obtained from Tabor's pJT122 plasmid, an illegal EcoR1 site is found. Uppsala-Sweden iGEM 2011 removed the site by carrying out point mutagenesis of A4C and A6C to obtain the part BBa_K592002. The part sequence was then subject to codon optimisation using the IDT codon optimisation tool to allow optimum expression of ccaR in the E.coli chassis. No new illegal restriction sites were introduced by this step.
Source
ccaR can be found in the genome of Synechocystis PCC 6803. The sequence can be obtained from BBa_K592002 (Uppsala-Sweden iGEM, 2011 - initially from Tabor's pJT122 plasmid). The part sequence was then fully optimised for our chosen chassis organism, E.coli, using the IDT codon optimisation tool.