Difference between revisions of "Part:BBa K3634008:Design"
(→Design Notes) |
(→References) |
||
(3 intermediate revisions by the same user not shown) | |||
Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
− | The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool. | + | The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool. |
===References=== | ===References=== | ||
+ | |||
+ | UTAustin iGEM 2004 - https://parts.igem.org/Part:BBa_I15009 | ||
+ | |||
+ | IDT Codon Optimisation Tool - https://eu.idtdna.com/CodonOpt | ||
+ | |||
+ | UniProtKB: Q55891 (PCYA_SYNY3) - https://www.uniprot.org/uniprot/Q55891 |
Latest revision as of 15:42, 5 August 2020
Phycocyanobilin:Ferredoxin Oxidoreductase (pcyA) (Optimised for E.coli)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence from BBa_I15009 was taken and subject to the IDT codon optimisation tool. During optimisation, no illegal restriction sites were created allowing for the part to be used at RFC[10] and RFC[1000] standard.
Source
The pcyA gene is found in Synechocystis sp. PCC 6803 and has been previously categorised in BioBrick form by J.J. Tabor (BBa_I15009). In order to maximise expression of phycocyanobilin:ferredoxin oxidoreductase in the chosen E.coli chassis, the St Andrews iGEM team of 2020 carried out codon optimisation using the IDT codon optimisation tool.
References
UTAustin iGEM 2004 - https://parts.igem.org/Part:BBa_I15009
IDT Codon Optimisation Tool - https://eu.idtdna.com/CodonOpt
UniProtKB: Q55891 (PCYA_SYNY3) - https://www.uniprot.org/uniprot/Q55891