Difference between revisions of "Part:BBa J119446"
 
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<partinfo>BBa_J119446 short</partinfo>
 
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The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. This sequence is found in Conditional Control of Suicide Gene Expression in Tumor Cells with Theophylline-Responsive Ribozyme (https://www.nature.com/articles/gt201678).
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The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).
  
 
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Latest revision as of 18:55, 18 June 2020


Mammal Ribozyme

The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]