Difference between revisions of "Part:BBa J119446"
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| − | The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. | + | The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Conditional Control of Suicide Gene Expression in Tumor Cells with Theophylline-Responsive Ribozyme (https://www.nature.com/articles/gt201678). |
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Revision as of 18:50, 18 June 2020
Mammal Ribozyme
The Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence is found in Conditional Control of Suicide Gene Expression in Tumor Cells with Theophylline-Responsive Ribozyme (https://www.nature.com/articles/gt201678).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]