Difference between revisions of "Part:BBa K3600003"
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* activator: Yap1p | * activator: Yap1p | ||
*GSH1 gene encodes gamma-glutamylcysteine synthetase.<html><a href="#Wu1994"><sup>1</sup></a></html> | *GSH1 gene encodes gamma-glutamylcysteine synthetase.<html><a href="#Wu1994"><sup>1</sup></a></html> | ||
− | *The promoter GSH1 has been chosen in order to express fluorescent protein [ref] when | + | *The promoter GSH1 has been chosen in order to express fluorescent protein [ref] when S.cervisiae is exposed to oxidative stress. |
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*The binding site for Yap1p, in the promoter GSH1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a> | *The binding site for Yap1p, in the promoter GSH1, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a> |
Revision as of 19:43, 14 June 2020
GSH1 promoter part plasmid
This part contains the promoter of the gene GSH1 from S. Cerevisiae.
Usage and Biology
- activator: Yap1p
- GSH1 gene encodes gamma-glutamylcysteine synthetase.1
- The promoter GSH1 has been chosen in order to express fluorescent protein [ref] when S.cervisiae is exposed to oxidative stress.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 975
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 563
Illegal BsaI site found at 972
Illegal BsaI.rc site found at 1084