Difference between revisions of "Part:BBa K3600001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
* activator: Yap1p | * activator: Yap1p | ||
| − | * | + | *TRX2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.<html><a href="#Kuge1994"><sup>1</sup></a></html> |
| − | *The promoter | + | *The promoter TRX2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress. |
| − | *The binding site for Yap1p, in the promoter | + | *The binding site for Yap1p, in the promoter TRX2, is TTACTAA.<html><a href="#Kuge1994"><sup>1</sup></a></html> |
*The yeast toolkit for modular assembly <html><a href="#Lee2015"><sup>2</sup></a></html> was used to assemble transcriptional units. This promoter was used as a part of type II. | *The yeast toolkit for modular assembly <html><a href="#Lee2015"><sup>2</sup></a></html> was used to assemble transcriptional units. This promoter was used as a part of type II. | ||
Revision as of 19:29, 14 June 2020
TRX2 promoter part plasmid
This part contains the promoter of the gene TRX2 from S. Cerevisiae.
Usage and Biology
- activator: Yap1p
- TRX2 gene encodes thioredoxin protein in Saccharomyces cerevisiae.1
- The promoter TRX2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to oxidative stress.
- The binding site for Yap1p, in the promoter TRX2, is TTACTAA.1
- The yeast toolkit for modular assembly 2 was used to assemble transcriptional units. This promoter was used as a part of type II.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 568
Illegal BsaI.rc site found at 1089